When John James Audubon first moved to America in 1803, he moved to Mill Grove near Valley Forge. It was in Pennsylvania that he developed his spectacular and unique painting style. In 1896, the Pennsylvania Audubon Society was created as the second state Audubon Society in the country.

In 1975, the National Audubon Society opened the Mid-Atlantic Regional Office near Harrisburg to serve Pennsylvania and five surrounding states.

National Audubon Society opened the Pennsylvania State Office on July 1, 1997 as the tenth state office created under Audubon's reorganization as directed by its new strategic plan.

Today, Pennsylvania has 22 Audubon chapters and more than 24,000 members.